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  • kristarockwell
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    These kanamycin resistant pUC vectors also contain a pUC19-derived multiple cloning site within the lacZ gene, enabling recombinant clones to be verified through culture plates containing IPTG and X-Gal. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors. High target gene expression is enabled by the presence of the lac promoter in both the pHSG298 and pHSG299 vectors … The purpose of the Public Utilities Commission is to regulate the electricity, water, and telecommunications sectors in Belize to efficiently provide the highest quality services at affordable rates, ensuring the viability and sustainability of each sector. The purpose of the Public Utilities Commission is to regulate the electricity, water, and telecommunications sectors in Belize to efficiently provide the highest quality services at affordable rates ensuring the viability and sustainability of each sector. The Hawaii Public Utilities Commission (“PUC”) initiated Phase 2 of the community based renewable energy program (“CBRE”) on April 9, 2020. The puc [lyksoomu.com]’s goal for Phase 2 is to dramatically expand access to the economic, environmental, and societal benefits of renewable energy. Our mission is to uplift communities through the creation of high quality public charter schools in which students are inspired and prepared to graduate from high school and university and commit to uplift their communities now and forever. Acremonium chrysogenum is the major producer of the β-lactam antibiotic cephalosporin C and therefore of great importance for the pharmaceutical industry. However, this filamentous fungus is known to reproduce solely by asexual means, shows only sporadic conidiospore production, and has gradual fragmentation of the vegetative mycelium into arthrospores. Due to these peculiar growth characteristics and life style, strain improvement by recombinant technologies is much more challenging than for other biotechnologically relevant fungi. Chrysogenum, including a ΔAcku70 deletion strain for homologous recombination. No physiological or morphological changes occurred due to deletion of the ku70 gene or integration of the nat1 cassette in this recipient strain. We also used a xylose-inducible promoter from Sordaria macrospora to demonstrate induction of the gfp reporter gene in A. The Smxyl promoter was used for construction of a vector molecule to develop a one-step FLP/FRT recombination system in A. This system was then used in the ΔAcku70 deletion strain to construct a marker-free recipient strain for targeted DNA insertion into genomic DNA.

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